CRISPR/Cas System and Factors Affecting Its Precision and Efficiency

CRISPR/Cas System and Factors Affecting Its Precision and Efficiency

Blog Article

The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas).The CRISPR/Cas system was originally discovered in bacteria as a part of adaptive-immune virginia mill works tobacco road acacia system with multiple types.Its engineered versions involve multiple host DNA-repair pathways in order to perform genome editing in host cells.However, it is still challenging to get maximum genome-editing efficiency click here with fewer or no off-targets.

Here, we focused on factors affecting the genome-editing efficiency and precision of CRISPR/Cas system along with its defense-mechanism, orthologues, and applications.